application and adaptation of dot immunoassay to detect and measure rubella virus antibody
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abstract
a dot immunobinding assay ( dia) was used for a quantitative and qualitative assay of rubella antibody. purified antigen and conjugated anti-human immunoglobulin (rahlg) were prepared. nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. the reacting antibodies were visualized by a peroxidase system. development of a colored insoluble substrate was taken as a positive result. the adapted dia was applied to test 105 serum samples. the sensitivity and specificity and immune titer of dia were compared with hemagglutination inhibition (hi) test.
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Journal title:
medical journal of islamic republic of iranجلد ۱۵، شماره ۳، صفحات ۱۷۱-۱۷۴
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